解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The steady-state oxidation of ferrocytochrome c by cytochrome oxidase monitored spectrophotometrically showed that: (1) the kinetics were strictly biphasic with purified enzyme, while mitochondrial membrane-bound enzyme exhibited multiphasic kinetics with extended low affinity phases; (2) the TNmax for the highest aff...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80321-4
更新日期:1983-12-12 00:00:00
abstract::The isolation of a novel biologically active peptide, designated galanin, is described. The peptide was discovered by the detection of its C-terminal amide structure in porcine intestinal extract using a chemical method. It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: Gly-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80033-7
更新日期:1983-11-28 00:00:00
abstract::Thymidine kinase in chick embryo retina reaches its highest values on the 8-10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocortic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80042-8
更新日期:1983-11-28 00:00:00
abstract::The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80035-0
更新日期:1983-11-28 00:00:00
abstract::We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80027-1
更新日期:1983-11-28 00:00:00
abstract::Tropomyosins can be classified as belonging to an alpha-like or a beta-like family depending on the absence or presence, respectively in their protease-V8 digestion pattern of two peptides with an apparent molecular mass of 21 kDa. Chicken cardiac tropomyosin and the 43 kDa component from gizzard tropomyosin are accor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80829-1
更新日期:1983-11-14 00:00:00
abstract::Vasopressin elicited a dose-dependent inhibition of glucagon-induced cAMP accumulation in isolated hepatocytes. This response was not diminished by incubation of cells with the calmodulin antagonists trifluoperazine or chlorpromazine and was only slightly reduced in Ca2+-depleted hepatocytes. Half-maximal inhibition o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80835-7
更新日期:1983-11-14 00:00:00
abstract::Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80822-9
更新日期:1983-11-14 00:00:00
abstract::Dipeptidylaminopeptidase IV, a plasma membrane-bound glycoprotein, is characterized by an intramolecular heterogeneous turnover of the protein backbone and carbohydrate chain. The faster turnover of the latter is restricted only to the outer sugars. The inner core sugars D-mannose and N-acetyl-D-glucosamine turn over ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81183-1
更新日期:1983-10-31 00:00:00
abstract::Three inositol-containing sialyloligosaccharides were isolated from normal human urine. Structural studies including gas-liquid chromatography of mono- and disaccharide derivatives, methylation analysis, mass spectrometry and glycosidase treatments indicated the structure NeuAc (alpha 2-3)Gal(beta 1-0)scyllo-inositol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81151-x
更新日期:1983-10-31 00:00:00
abstract::Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81164-8
更新日期:1983-10-31 00:00:00
abstract::The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80803-5
更新日期:1983-10-17 00:00:00
abstract::The Na+-induced release of accumulated Ca2+ from heart mitochondria is inhibited by amiloride, benzamil and several other amiloride analogues. These drugs do not affect uptake or release of Ca2+ mediated by the ruthenium red-sensitive uniporter and their effects, like those of diltiazem and other Ca2+-antagonists, app...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80768-6
更新日期:1983-10-17 00:00:00
abstract::Platelet release products and purified platelet-derived growth factor stimulated the phosphorylation of ribosomal protein S6 in cultured mouse Balb/c 3T3 fibroblasts. The post-nuclear fraction of the stimulated cells was enriched in S6 kinase activity specific for sites resembling those phosphorylated within intact ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81072-2
更新日期:1983-10-03 00:00:00
abstract::32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81073-4
更新日期:1983-10-03 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80725-x
更新日期:1983-09-05 00:00:00
abstract::In washed anterior pituitary membranes, there is enough GTP to occupy Ns and therefore to obtain activation of adenylate cyclase by vasointestinal peptide. GTP concentrations needed to obtain adenylate cyclase inhibition by dopamine (above 5 X 10- M) stimulate the adenylate cyclase. The dopamine effect is a blockade o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80741-8
更新日期:1983-09-05 00:00:00
abstract::Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80732-7
更新日期:1983-09-05 00:00:00
abstract::Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80946-6
更新日期:1983-08-22 00:00:00
abstract::A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80942-9
更新日期:1983-08-22 00:00:00
abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80964-8
更新日期:1983-08-22 00:00:00
abstract::We have used 19F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca2+ with Ka greater than 4 X 10(4) M in terms of 19F NMR of TFP and that KCl markedly influences the interaction of TFP with Ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80962-4
更新日期:1983-08-22 00:00:00
abstract::Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80948-x
更新日期:1983-08-22 00:00:00
abstract::The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80971-5
更新日期:1983-08-22 00:00:00
abstract::The rate of formation of diferric-transferrin has been studied using various combinations of Fe(II), Tf, Cp and h serum. When the reactants were added in a correct physiological sequence, ceruloplasmin and diluted human serum showed the fastest rate of saturation of transferrin. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80432-3
更新日期:1983-08-08 00:00:00
abstract::Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80410-4
更新日期:1983-08-08 00:00:00
abstract::The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80420-7
更新日期:1983-08-08 00:00:00
abstract::We report that glycolysis in human quiescent fibroblasts stimulated by serum addition is increased, and that the changes of the metabolic route reflect the activity of the phosphofructokinase. A possible role of fructose-2,6-bisphosphate as a positive modulator of the key enzyme is proposed. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80412-8
更新日期:1983-08-08 00:00:00
abstract::Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80449-9
更新日期:1983-08-08 00:00:00
abstract::Crosslinking of histones in mouse liver nuclei and extended chromatin with a bifunctional reagent leads to the formation of H1-H1o heterodimers as well as H1o-H1o homodimers. H1o can be also crosslinked to the core histones. Thus, the location of histone H1o within the basic repeating chromatin structure seems to be a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80594-8
更新日期:1983-07-25 00:00:00
abstract::Eleven cloned hybridomas which secrete antibodies to acetylcholine receptors from human muscle have been prepared. All of these monoclonal antibodies to have the same basic specificity as shown by competition for binding to the main immunogenic region on the receptor, but these antibodies differ in fine specificity as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80688-7
更新日期:1983-07-11 00:00:00
abstract::Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80689-9
更新日期:1983-07-11 00:00:00
abstract::Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80690-5
更新日期:1983-07-11 00:00:00
abstract::To gain an understanding of the mechanism by which the hydroxyl free radical can arise in superoxide generating systems and learn how different chelaters of iron can inhibit this reaction, a pulse radiolysis kinetic study of the reaction of O;- with Fe(III)EDTA, Fe(III)HEDTA and Fe(III)DETAPAC (or DTPA)was undertaken....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80695-4
更新日期:1983-07-11 00:00:00
abstract::Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80672-3
更新日期:1983-07-11 00:00:00
abstract::The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80574-2
更新日期:1983-07-04 00:00:00
abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81130-2
更新日期:1983-06-27 00:00:00
abstract::Branched-chain 2-oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81115-6
更新日期:1983-06-27 00:00:00
abstract::Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81128-4
更新日期:1983-06-27 00:00:00